Effects of Fe3O4 nanoparticles on the transcription of genes with iron-responsive elements

Author

Liu, Yingxun ; Wang, Jinke

Author_Institution

State key Lab. of Bioelectronics, Southeast Univ., Nanjing, China

fYear

2012

Firstpage

1

Lastpage

4

Abstract

In this paper, we checked the transcription of 24 murine genes containing iron-responsive elements in Fe₃O₄ magnetic nanoparticles treated mouse macrophage RAW264.7 cells using cDNA microarrays and real time-PCR. The results showed that only 7 genes differentially expressed (fold change <;-1.5 or >; 1.5) after incubated with 100 μg/ml Fe₃O₄ magnetic nanoparticles. Gene Ontology analysis of these genes containing iron-responsive elements demonstrated that the differentially expressed genes, including Tfrc, Slc11a2 and Slc40a1, played a crucial role in iron ion homeostasis. And the non-differentially expressed genes (-1.5<; fold change <;1.5) were related to cell biosynthesis and metabolism, including acetyl-CoA metabolic process, peptide metabolic process, and macromolecule catabolic process.

Keywords

biochemistry; cellular biophysics; genetics; iron compounds; lab-on-a-chip; macromolecules; magnetic particles; nanomedicine; nanoparticles; Fe₃O₄; Fe₃O₄ magnetic nanoparticles; RAW264.7 cells; Slc11a2; Slc40a1; Tfrc; acetyl-CoA metabolic process; cDNA microarrays; cell biosynthesis; gene ontology analysis; gene transcription; iron ion homeostasis; iron-responsive elements; macromolecule catabolic process; metabolism; mouse macrophage; murine genes; peptide metabolic process; real time-PCR; Gene expression; Iron; Magnetic resonance imaging; Nanoparticles; Peptides; Proteins;

fLanguage

English

Publisher

ieee

Conference_Titel

Nanotechnology (IEEE-NANO), 2012 12th IEEE Conference on

Conference_Location

Birmingham

ISSN

1944-9399

Print_ISBN

978-1-4673-2198-3

Type

conf

DOI

10.1109/NANO.2012.6321938

Filename

6321938