Analysis of urea in human serum using an oblique angle deposited Ag nanorod surface enhanced Raman scattering substrate

Author

Han, Yun-ah ; Ju, Jonghyun ; Yoon, Yoosik ; Kim, Seok-min

Author_Institution

Sch. of Mech. Eng., Chung-Ang Univ., Seoul, South Korea

fYear

2012

Firstpage

1

Lastpage

4

Abstract

We applied surface enhanced Raman spectroscopy (SERS) for the detection of urea in human serum. An oblique angle deposition method was used to prepare SERS-active substrates consisting of Ag nanorods. To maximize the SERS enhancement, the effects of Ag nanorod length on the SERS signal were analyzed. The SERS signals of different concentrations of urea solutions were measured in order to generate a regression model for use in analyzing the amount of urea in body fluid using the SERS substrate. To examine the feasibility of the fabricated SERS substrate, the amount of urea in human serum was measured using the SERS substrate and compared with that determined via conventional blood analysis.

Keywords

biochemistry; biological techniques; biomedical measurement; blood; molecular biophysics; nanofabrication; nanorods; physiological models; proteins; regression analysis; silver; surface enhanced Raman scattering; Ag; SERS signals; SERS-active substrates; body fluid; conventional blood analysis; human serum; oblique angle deposited nanorod; oblique angle deposition method; regression model; surface enhanced Raman scattering substrate; urea analysis; urea detection; urea solution concentrations; Biochemistry; Humans; Monitoring; Photonics; Surface treatment;

fLanguage

English

Publisher

ieee

Conference_Titel

Nanotechnology (IEEE-NANO), 2012 12th IEEE Conference on

Conference_Location

Birmingham

ISSN

1944-9399

Print_ISBN

978-1-4673-2198-3

Type

conf

DOI

10.1109/NANO.2012.6321979

Filename

6321979