Title :
Effect of buffer concentration for the dynamics of DNA stretching with the molecular combing
Author :
Yuanyuan Yang ; Kaige Wang ; Qiwei Qiao ; Dan Li ; Shuang Wang ; Zhaoyu Ren ; Jintao Bai
Author_Institution :
Nat. Key Lab. of Photoelectric Technol. & Functional Mater., Northwest Univ., Xi´an, China
Abstract :
This paper experimented with the λ-DNA molecules which were successfully stretched on the hydrophobic surface of PMMA by the molecular combing method in a wide concentration range from 7mM to 20mM of tris buffer. The buffer concentration has a remarkable influence on the properties of the stretched DNA such as the length and uniformity. The results demonstrated the most applicable concentration of tris buffer for λ-DNA stretching is 10mM.
Keywords :
DNA; hydrophobicity; molecular biophysics; molecular configurations; λ-DNA molecules; DNA stretching dynamics; PMMA; buffer concentration; hydrophobic surface; molecular combing method; tris buffer; DNA; Lighting; Nanoscale devices; buffer concentration; dynamics; molecule combing; single DNA molecule;
Conference_Titel :
Nanotechnology (IEEE-NANO), 2012 12th IEEE Conference on
Conference_Location :
Birmingham
Print_ISBN :
978-1-4673-2198-3
DOI :
10.1109/NANO.2012.6321980
