Quantum dot FRET linker probes for highly sensitive DNA methylation detection

DNA methylation is an important cancer biomarker, but improving the detection sensitivity of DNA methylation remains a challenge for various types of biological samples. Here, we describe a novel quantum dot (QD)-based method using FRET linker probes (FLPs) that further increases the sensitivity of detection of DNA methylation patterns. This method relies on removing the background noise resulting from PCR inefficiencies. Compared to conventional non-specific QD-FRET detection, we demonstrate the improved detection of DNA methylation by fluorescence detection in bulk and with single molecule resolution.