Title :
Evaporation-driven microfluidic sample concentration
Author :
Walker, G.M. ; Beebe, D.J.
Author_Institution :
Dept. of Biomed. Eng., Wisconsin Univ., Madison, WI, USA
fDate :
6/24/1905 12:00:00 AM
Abstract :
A new method for sample concentration within microfluidic devices is presented. Evaporation at an air/liquid interface is used to induce flow. within a microfluidic device and concentrate sample. The practicality of this method was demonstrated with 0.2 μm fluorescent microspheres and FITC-labeled bovine serum albumin (BSA). Eighty one percent of a 0.6 μL fluorescent sphere suspension was concentrated into a well within a microfluidic device. In the same amount of time, 93% of a 0.6 μL FITC-labeled BSA solution was concentrated
Keywords :
biological techniques; evaporation; mass transfer; microfluidics; separation; suspensions; air/liquid interface; average velocities; bovine serum albumin; concentration gradient; equivalent volumetric flow rates; evaporation-driven; fluorescent microspheres; mass transfer coefficient; microfluidic devices; polydimethylsiloxane mold; rapid prototyping; sample concentration; straight micro fluidic. channel; Biological system modeling; Biomedical engineering; Fluorescence; Microchannel; Microfluidics; Reservoirs; Solids; Stacking; Surface treatment; Water resources;
Conference_Titel :
Microtechnologies in Medicine & Biology 2nd Annual International IEEE-EMB Special Topic Conference on
Conference_Location :
Madison, WI
Print_ISBN :
0-7803-7480-0
DOI :
10.1109/MMB.2002.1002397
