Title :
Combined Photoacoustic and Molecular Fluorescence Imaging In Vivo
Author :
Wang, Lihong V. ; Xie, Xueyi ; Oh, Jung-Taek ; Li, Meng-Lin ; Ku, Geng ; Ke, Shi ; Similache, Sergiu ; Li, Chun ; Stoica, George
Author_Institution :
Dept. of Biomed. Eng., Texas A&M Univ., College Station, TX
Abstract :
Because of the overwhelming scattering of light in biological tissues, the spatial resolution and imaging depth of conventional fluorescent imaging is unsatisfactory. Therefore, we present a dual modality imaging technique by combining fluorescence imaging with high-resolution noninvasive photoacoustic tomography (PAT) for the study of an animal tumor model. PAT provides high-resolution structural images of tumor angiogenesis, and fluorescence imaging offers high sensitivity to molecular probes for tumor detection. Coregistration of the PAT and fluorescence images was performed on nude mice with M21 human melanoma cell lines with alphavbeta3 integrin expression. An integrin alphavbeta3-targeted peptide-ICG conjugated NIR fluorescent contrast agent was used as the molecular probe for tumor detection. PAT was employed to noninvasively image the brain structure and the angiogenesis associated with tumors in mice. The coregistration between the PAT and fluorescence images was used to visualize tumor location, angiogenesis, and brain structure simultaneously
Keywords :
biomedical optical imaging; biomedical ultrasonics; cellular biophysics; fluorescence; image registration; image resolution; medical image processing; molecular biophysics; proteins; tumours; M21 human melanoma cell lines; angiogenesis; animal tumor model; biological tissues; brain structure; dual modality imaging; fluorescence imaging; fluorescent contrast agent; high-resolution structural images; high-resolution tomography; imaging depth; integrin; light scattering; molecular fluorescence imaging; molecular probes; near infrared contrast agent; noninvasive tomography; peptide-ICG; photoacoustic imaging; photoacoustic tomography; spatial resolution; tumor angiogenesis; tumor detection; tumor location; Biological tissues; Brain; Fluorescence; High-resolution imaging; In vivo; Light scattering; Mice; Neoplasms; Probes; Tumors;
Conference_Titel :
Engineering in Medicine and Biology Society, 2005. IEEE-EMBS 2005. 27th Annual International Conference of the
Conference_Location :
Shanghai
Print_ISBN :
0-7803-8741-4
DOI :
10.1109/IEMBS.2005.1616374