Title :
Fluorescence anisotropy of protein - Gold nanoclusters
Author :
Li, Chenhao ; Sutter, Jens U. ; Birch, David J S ; Chen, Yu
Author_Institution :
Dept. of Phys., Strathclyde Univ., Glasgow, UK
Abstract :
Red-emitting gold nanoclusters (BSA-Au25) have been synthesized in Bovine Serum albumin and found to have a characteristic fluorescence lifetime of 1.25μs. Fluorescence anisotropy measurements of BSA-Au25 reveal a dramatic increase in protein size as the pH is reduced from 7 to 3.1, consistent with the change of BSA from N form to E form. This study suggests the possibility of exploiting fluorescent protein-gold nanoclusters to probe protein conformational changes in response to the local environment.
Keywords :
atomic clusters; biological techniques; fluorescence; fluorescence spectroscopy; gold; molecular biophysics; molecular configurations; nanobiotechnology; nanoparticles; pH; proteins; radiative lifetimes; Au25; BSA-Au25; bovine serum albumin; fluorescence anisotropy; fluorescence lifetime; pH 3.1; pH 7; protein conformational changes; protein-gold nanoclusters; red emitting gold nanoclusters; time 1.25 mus; Color; Detectors; Fluorescence; Gold; Green products; Probes; Proteins; BSA; fluorescence anisotropy; gold nanoclusters; protein conformational change;
Conference_Titel :
Nanotechnology (IEEE-NANO), 2012 12th IEEE Conference on
Conference_Location :
Birmingham
Print_ISBN :
978-1-4673-2198-3
DOI :
10.1109/NANO.2012.6322067
