Dielectric response of cancer cells after exposure to single and multiple nanosecond pulses

Intense nanosecond pulsed electric fields (nsPEFs) can cause various effects in biological cells and tissues. The quality and evolution of induced membrane poration phenomena are essential for the ultimate fate of cells. Recent Molecular Dynamics (MD) simulations have predicted that the poration process is a two-fold process and that nanopores formed by a nsPEF-exposure can be long-lived [1]. To better understand the role of long-lived nanopores in nsPEF-cell interactions, we have investigated the dielectric response of Jurkat cells after exposure to single and multiple 300-ns pulses of similar dose of electrical energy by means of time domain dielectric spectroscopy.