Title :
Self-assembling of DNA and PAMAM dendrimer
Author :
Zhou, L.Z. ; Yang, X.L. ; Gan, L. ; Xu, H.B.
Author_Institution :
Coll. of Life Sci. & Tech., Huazhong Univ. of Sci. & Tech., Wuhan
Abstract :
The self-assembling of DNA and cationic polymers are of interest for their biological applications. In this paper, the interaction of DNA with PAMAM was studied by agarose gel electrophoresis, size and zeta-potential measurements and circular dichroism (CD) spectroscopy. DNA was shown to form stable complexes with PAMAM by gel electrophoresis. The mean particle size of the complexes was in the range of 150-180 nm. The zeta-potential was positive and increased with an increase of N/P ratios. Compared to naked DNA, CD spectra of complexed DNA was changed and had a decreased intensity at 270 nm. High salts and excessively low or high pH values could also result in decreased CD signals of PAMAM complexed DNA. These results provide some instructions for assembling of DNA as well as gene therapy applications
Keywords :
DNA; biochemistry; bioelectric phenomena; circular dichroism; electrokinetic effects; electrophoresis; molecular biophysics; pH; particle size; polymers; self-assembly; 150 to 180 nm; DNA; PAMAM dendrimer; agarose gel electrophoresis; cationic polymers; circular dichroism spectroscopy; gene therapy; pH; particle size; self-assembly; zeta-potential measurements; Chemical technology; DNA; Electrokinetics; Electrostatics; Gene therapy; Materials science and technology; Polymers; Size measurement; Spectroscopy; Temperature;
Conference_Titel :
Engineering in Medicine and Biology Society, 2005. IEEE-EMBS 2005. 27th Annual International Conference of the
Conference_Location :
Shanghai
Print_ISBN :
0-7803-8741-4
DOI :
10.1109/IEMBS.2005.1616456
