Title :
Highly Effective In Vivo Gene Transfection by Blocked Star Vector
Author :
Zhou, Y.M. ; Huang, H. ; Nakayama, Y.
Author_Institution :
Dept. of Bioeng., Nat. Cardiovascular Center Res. Inst., Osaka
Abstract :
Nano-structured four branched, blocked star vector (BSV) was molecularly designed by iniferter-based photo-living radical copolymerization method of N,N-dimethylaminopropylacrylamide and then N,N-dimethylacrylamide from four dithiocarbamate-derivatized benzene for a novel gene delivery non-viral vector. The conjugated pDNA complexes were satisfactorily stable in the condition of the electrophoresis. Under the charge ratio of 5/1 (vector/pDNA), complexes of BSV and pDNA (BSV-pDNA) demonstrated satisfied transfection efficiency into COS-1 cells with a little cytotoxicity. Mice injected with BSV-pDNA polyplexes showed a higher level of gene expression in either liver, kidney or spleen using the optimal charge ratio as determined in in vitro. These results suggest the potential use of BSV as a non-viral vector in clinic
Keywords :
DNA; bioelectric phenomena; cellular biophysics; electrophoresis; genetics; kidney; liver; molecular biophysics; nanostructured materials; nanotechnology; patient treatment; polymer blends; polymerisation; BSV-pDNA polyplexes; COS-1 cells; N,N-dimethylacrylamide; N,N-dimethylaminopropylacrylamide; conjugated pDNA complexes; cytotoxicity; dithiocarbamate-derivatized benzene; electrophoresis; gene delivery nonviral vector; gene expression; highly effective in vivo gene transfection; iniferter-based photo-living radical copolymerization method; kidney; liver; mice; nanostructured four branched blocked star vector; spleen; Biomedical engineering; Cardiology; DNA; Electrokinetics; Gene expression; In vitro; In vivo; Mice; Nanobioscience; Polymers;
Conference_Titel :
Engineering in Medicine and Biology Society, 2005. IEEE-EMBS 2005. 27th Annual International Conference of the
Conference_Location :
Shanghai
Print_ISBN :
0-7803-8741-4
DOI :
10.1109/IEMBS.2005.1616457
