Title :
Spectroscopic characteristics and cellular compatibility of protein wrapped single wall carbon nanotubes
Author :
Bertulli, Cristina ; Beeson, Harry J. ; Hasan, Tawfique ; Ferrari, Andrea C. ; Huang, Yan Yan S
Author_Institution :
Cavendish Lab., Univ. of Cambridge, Cambridge, UK
Abstract :
Non-covalent functionalization of CoMoCAT single-wall carbon nanotubes (SWNTs) by bovine serum albumin (BSA) was achieved. Photoluminescence spectra for the functionalized nanotubes showed good dispersion by BSA functionalization. Raman spectra were taken for the sonicated SWNT-BSA solution to establish the signal versus concentration correlation. Cellular uptake of functionalized carbon nanotubes by mouse macrophage (RAW264.7) was then investigated using Raman spectroscopy. For a seeding density of 50% confluence in a culture solution containing 10 μg/ml of BSA-SWNTs, uptake of ~200 μg/ml by the macrophages was recorded after 23hr incubation, indicating an active uptake of SWNTs.
Keywords :
Raman spectroscopy; carbon nanotubes; cellular biophysics; photoluminescence; proteins; BSA functionalization; C; CoMoCAT single-wall carbon nanotube; Raman spectra; bovine serum albumin; cellular compatibility; cellular uptake; functionalized carbon nanotube; functionalized nanotube; incubation; mouse macrophage; non-covalent functionalization; photoluminescence spectra; protein wrapped single wall carbon nanotube; seeding density; sonicated SWNT-BSA solution; spectroscopic characteristics; time 23 h; Carbon nanotubes; Mice; Probes; Proteins; Ultrasonic variables measurement;
Conference_Titel :
Nanotechnology (IEEE-NANO), 2012 12th IEEE Conference on
Conference_Location :
Birmingham
Print_ISBN :
978-1-4673-2198-3
DOI :
10.1109/NANO.2012.6322192
