The Biological Effect of the NanoTiO2and Its Toxicity

The proliferation behavior of the person´s liver cell under actions of the nanoTiO₂ has been looked into by 3-(4,5-dimethylthiazol 2-yl)-2,5 diphenyltetrazolium bromide (MTT) colorimetric method of Mosmann. In this case we first give some elementary properties of the nanoTiO₂ and do further the liver cell cultures, and add the nano TiO₂ into the cells. In accordance with biological rules we measure the proliferation state of the liver cell in normal condition. The result obtained show that the influence of the nanoTiO₂ on the proliferation of the person´s liver cell is very small, when compared with that of the control group. We found further out the size of the toxicity of the nanoTiO₂ to the chick embryo fibroblast (CEF) cells by the MTT colorimetric method. Thus we determine the toxicity for the TiO₂s of 100 nm and of 1000 nm by the toxicology which are all first score (or degree)