Title :
The Biological Effect of the NanoTiO2and Its Toxicity
Author :
Xiao-feng, Pang ; Lewei, Liu ; Zhi Hong, Liu ; Qiang, Zhao
Author_Institution :
Inst. of Life Sci. & Technol., Univ. of Electron. Sci. & Technol. of China, Chengdu
Abstract :
The proliferation behavior of the person´s liver cell under actions of the nanoTiO2 has been looked into by 3-(4,5-dimethylthiazol 2-yl)-2,5 diphenyltetrazolium bromide (MTT) colorimetric method of Mosmann. In this case we first give some elementary properties of the nanoTiO2 and do further the liver cell cultures, and add the nano TiO2 into the cells. In accordance with biological rules we measure the proliferation state of the liver cell in normal condition. The result obtained show that the influence of the nanoTiO2 on the proliferation of the person´s liver cell is very small, when compared with that of the control group. We found further out the size of the toxicity of the nanoTiO2 to the chick embryo fibroblast (CEF) cells by the MTT colorimetric method. Thus we determine the toxicity for the TiO2s of 100 nm and of 1000 nm by the toxicology which are all first score (or degree)
Keywords :
cellular biophysics; colorimetry; liver; nanostructured materials; titanium compounds; toxicology; 3-(4,5-dimethylthiazol 2-yl)-2,5 diphenyltetrazolium bromide (MTT) colorimetric method; TiO2; chick embryo fibroblast cells; liver cell proliferation; nanoTiO2 biological effect; toxicity; toxicology; Agriculture; Cells (biology); Government; Liver; Marketing and sales; Nanobioscience; Nanomaterials; Nanotechnology; Production facilities; Wavelength measurement; MTT colorimetric method; biological effect; chick embryo fibroblast (CEF) cell; nanoTiO; person´s liver cell; proliferation; toxicity;
Conference_Titel :
Engineering in Medicine and Biology Society, 2005. IEEE-EMBS 2005. 27th Annual International Conference of the
Conference_Location :
Shanghai
Print_ISBN :
0-7803-8741-4
DOI :
10.1109/IEMBS.2005.1616650
