Fabricating nanofluidic channels and Applying it for single bio-molecule study

In the emerging field of nanobiotechnology, further downsizing the fluidic channels to the nanometer scale is attractive for both fundamental studies and technical applications. The insulation silicon nitride membrane nanofluidic channel array which have width ~75 nm and depth ~100 nm and length 50 mum were created by focused-ion-beam instrument, the lambda-DNA molecules were put inside them and the dynamic characteristics were initial studied, a fluorescence microscopy was used to observe the images. We observed lambda-DNA moved inside the nanotrenches which dealt with activating reagent Brij aqueous solution only by capillary force, this will help us to understand more DNA dynamics characteristics and more information about single biomolecule transporting through a nanopore