A glucose oxidase sensor based on screen-printed carbon electrodes modified by polypyrrole

Author

Xu, Hui ; Li, Guang ; Wu, Jieying ; Wang, You ; Liu, Jun

Author_Institution

Dept. of Biomed. Eng., Zhejiang Univ., Hangzhou

fYear

2006

Firstpage

1917

Lastpage

1920

Abstract

A disposable amperometric biosensor for detecting blood glucose has been developed. The sensor is based on a screen-printed electrode prepared by electrochemical polymerization of pyrrole with glucose oxidase (GOD) and LiClO₄ dopants. In citric acid buffer (pH5.0), GOD with negative charges is immobilized within electropositive polypyrrole matrices onto a carbon electrode surface. Afterward, the electron transfer mediator, potassium ferricyanide is immobilized by adsorption. Experimentally the compositions of pyrrole, LiClO₄ and potassium ferricyanide are optimized. Amperometry is used for the determination of glucose concentration. Four microliters of glucose solution is needed for one test, and the response time of the sensor is 70s. The amperometric response of the enzyme electrode is linear in the range of 1-30 mM

Keywords

amperometric sensors; biochemistry; biosensors; blood; carbon; electrochemical electrodes; electrodes; enzymes; lithium compounds; molecular biophysics; polymers; 70 s; LiClO₄ dopants; adsorption; blood glucose detection; citric acid buffer; disposable amperometric biosensor; electrochemical polymerization; electron transfer mediator; electropositive polypyrrole matrices; enzyme electrode; glucose oxidase sensor; glucose solution; potassium ferricyanide; screen-printed carbon electrodes; Biochemistry; Biomedical electrodes; Biomedical engineering; Biosensors; Electrons; Ink; Polymers; Silver; Sugar; Temperature;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 2005. IEEE-EMBS 2005. 27th Annual International Conference of the

Conference_Location

Shanghai

Print_ISBN

0-7803-8741-4

Type

conf

DOI

10.1109/IEMBS.2005.1616826

Filename

1616826