Title :
A glucose oxidase sensor based on screen-printed carbon electrodes modified by polypyrrole
Author :
Xu, Hui ; Li, Guang ; Wu, Jieying ; Wang, You ; Liu, Jun
Author_Institution :
Dept. of Biomed. Eng., Zhejiang Univ., Hangzhou
Abstract :
A disposable amperometric biosensor for detecting blood glucose has been developed. The sensor is based on a screen-printed electrode prepared by electrochemical polymerization of pyrrole with glucose oxidase (GOD) and LiClO4 dopants. In citric acid buffer (pH5.0), GOD with negative charges is immobilized within electropositive polypyrrole matrices onto a carbon electrode surface. Afterward, the electron transfer mediator, potassium ferricyanide is immobilized by adsorption. Experimentally the compositions of pyrrole, LiClO4 and potassium ferricyanide are optimized. Amperometry is used for the determination of glucose concentration. Four microliters of glucose solution is needed for one test, and the response time of the sensor is 70s. The amperometric response of the enzyme electrode is linear in the range of 1-30 mM
Keywords :
amperometric sensors; biochemistry; biosensors; blood; carbon; electrochemical electrodes; electrodes; enzymes; lithium compounds; molecular biophysics; polymers; 70 s; LiClO4 dopants; adsorption; blood glucose detection; citric acid buffer; disposable amperometric biosensor; electrochemical polymerization; electron transfer mediator; electropositive polypyrrole matrices; enzyme electrode; glucose oxidase sensor; glucose solution; potassium ferricyanide; screen-printed carbon electrodes; Biochemistry; Biomedical electrodes; Biomedical engineering; Biosensors; Electrons; Ink; Polymers; Silver; Sugar; Temperature;
Conference_Titel :
Engineering in Medicine and Biology Society, 2005. IEEE-EMBS 2005. 27th Annual International Conference of the
Conference_Location :
Shanghai
Print_ISBN :
0-7803-8741-4
DOI :
10.1109/IEMBS.2005.1616826
