Polypyrrole Based Reporterless DNA/Protein Sensors

Polypyrrole (PPy) is one of the important conductive polymers that are widely used in energy storage systems, biosensors and electronics. The electrochemical synthesis of PPy has advantages of simple process, mass production and low cost. In this study, polypyrolle were used to reporterlessly detect DNA and proteins. PPy thin films impregnated with probe molecules were deposited on both microelectrode and glassy carbon electrode surface by galvanostatic deposition method. Results demonstrated that PPy coated electrodes can be used for successful detection of DNA hybridization and antibody (Ab)-antigen (Ag) bindings. The experimental results also showed that the detection mechanism is probably due to the change of doping/undoping process of the conductive polymer after the DNA hybridization or antibody/antigen bindings