Title :
Measurement of bioimpedance and cell viability during ischemia-reperfusion in the rat liver
Author :
Ahn, H.S. ; Yun, S.S. ; Shin, H.J. ; Kim, J.Y. ; Choi, J.H.
Author_Institution :
Dept. of Biomed. Eng., Yeungnam Univ., Daegu
Abstract :
During liver resection and liver transplant, liver is damaged by ischemia-reperfusion injury. Until now, there is no approved method to measure or predict the extent of liver injury during the operation. This is the preliminary study to make the real time monitoring system by quantification of bioimpedance and ischemia-reperfusion injury in liver. Sprague-Dawley rats were subjected to different periods of 70% partial hepatic ischemia (30, 60, 90 and 120 minutes ischemia) and reperfusion. We measured changes of liver tissue bioimpedance (120 Hz - 100 KHz) every five minutes. Cell viability was assessed by metabolic capacity of fatty acid (palmitic acid metabolic rate), ATP content and histological examination (H/E and TUNEL stain) at every 30 minutes interval during ischemia
Keywords :
bioelectric phenomena; biological tissues; biomedical measurement; cellular biophysics; haemorheology; liver; patient monitoring; 120 Hz to 100 kHz; 120 min; 30 min; 60 min; 90 min; ATP content; Sprague-Dawley rats; cell viability; fatty acid; histological examination; ischemia-reperfusion; liver resection; liver tissue bioimpedance; liver transplant; metabolic capacity; palmitic acid metabolic rate; partial hepatic ischemia; real time monitoring system; Bioimpedance; Biological materials; Biomedical measurements; Blood; Conducting materials; Frequency; Injuries; Ischemic pain; Liver; Rats; bioimpedance; ischemia-reperfusion; liver;
Conference_Titel :
Engineering in Medicine and Biology Society, 2005. IEEE-EMBS 2005. 27th Annual International Conference of the
Conference_Location :
Shanghai
Print_ISBN :
0-7803-8741-4
DOI :
10.1109/IEMBS.2005.1616833
