An optimizing operator of auto-focusing algorithm for splicing graphics with microscope system

An optimizing operator of focusing algorithms is proposed for automatic microscope system. Based on the characteristics of optimal evaluation function, this operator is applicable to automatic splicing graphics with microscope system. Experiments show that the optimized algorithm not only becomes much sharper near the focusing point, but also changes more slowly when it is far from the focusing point. Therefore, the signal to noise ratio (S/N) is largely improved and it becomes much easier to focus. On the other hand, the operator needs only a few operations of addition and multiplication. This optimizing operator obviously improved both the precision and the speed in our system for automatic splicing graphics.