Title :
QCM-Based DNA biosensor for Salmonella typhimurium detection
Author :
Waiwijit, U. ; Jaruwongrungsee, K. ; Chokesajjawatee, N. ; Promjai, J. ; Lomas, T. ; Sritongkham, Pompimol ; Tuantranont, Adisorn
Author_Institution :
MEMS Lab., Nat. Electron. & Comput. Technol. Center (NECTEC), Pathumthani, Thailand
Abstract :
This work demonstrated a QCM biosensor for the detection of Salmonella typhimurium using the specific DNA probe. A thiol DNA probe was immobilized onto quartz surface coated with gold through self-assembly via Au-S bonding formation. 6-mercapto-1-hexanol (MCH) was used as blocking reagent against non-specific DNA binding. Target DNA with 94 bp (single strand) was hybridized with its probe resulted in a decrease in the resonance frequency of the QCM biosensor. It was demonstrated that the QCM biosensor could recognize the target DNA sequence within 40-70 min. The frequency shift signal was distinguishable along with the increase of target concentration from 0.1 to 1.0 μM.
Keywords :
DNA; biochemistry; biosensors; cellular biophysics; chemical sensors; gold; microorganisms; molecular biophysics; organic compounds; quartz; self-assembly; 6-mercapto-1-hexanol; QCM-based DNA biosensor; blocking reagent; frequency shift signal; gold-sulphur bonding formation; nonspecific DNA binding; quartz surface coating; resonance frequency; salmonella typhimurium detection; self-assembly; single strand DNA; target DNA sequence; target concentration; thiol DNA probe; time 40 min to 70 min; Biosensors; Crystals; DNA; Electrodes; Gold; Microorganisms; Probes; DNA biosensor; QCM; Salmonella typhimurium;
Conference_Titel :
Electron Devices and Solid State Circuit (EDSSC), 2012 IEEE International Conference on
Conference_Location :
Bangkok
Print_ISBN :
978-1-4673-5694-7
DOI :
10.1109/EDSSC.2012.6482844
