DocumentCode
162520
Title
Homology Modeling of Receptor Kinase (SERK) Enzyme from Embryogenesis of Endosperms
Author
Ravi, D. ; Santhi, S. ; Parthasarathy, R. ; Vijayabharathi, V.
Author_Institution
Dept. of Botany, Gov. Arts Coll., Coimbatore, India
fYear
2014
fDate
6-7 March 2014
Firstpage
11
Lastpage
17
Abstract
The Insilco analysis of SERK (Somatic Embryogenesis Receptor Kinase) gene which helps in the ovulation time of plantation crops [14, 15] with thus evidence, the current study was preceded in Cocos nucifera. The SERK gene was retrieved from NCBI and appropriate protein sequence alignment for this protein sequence was done, for finding the evolutionary relationship of the SERK in Cocos nucifera and other plantation crops. A phylogenetic tree was constructed and it was found that there is more similarity for Cocos nucifera towards Arabidopsis thaliana. Primers were designed based on conserved regions of SERK genes reported from other plant species and then they were amplificated by using PCR technique. Homology modeling was done for the CnSERK, Where leucine rich repeats and serine/threonine regions was found, this gives more information regarding the function of SERK protein.
Keywords
bioinformatics; crops; enzymes; evolution (biological); genetics; Arabidopsis thaliana; CnSERK; Cocos nucifera; NCBI; PCR technique; SERK gene; SERK protein; embryogenesis; endosperms; evolutionary relationship; homology modeling; leucine rich repeats; ovulation time; phylogenetic tree; plant species; plantation crops; protein sequence alignment; receptor kinase enzyme; serine region; somatic embryogenesis receptor kinase gene; threonine region; Amino acids; Analytical models; Phylogeny; Protein sequence; Software; Solid modeling; Cocos nucifera; FAST PCR; GOR; Homology modeling; Interpro analysis; NCBI; Primer designing; SERK; SWISSPDB;
fLanguage
English
Publisher
ieee
Conference_Titel
Intelligent Computing Applications (ICICA), 2014 International Conference on
Conference_Location
Coimbatore
Type
conf
DOI
10.1109/ICICA.2014.12
Filename
6965002
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