Immunosensor for detecting pulmonary tuberculosis markers in human serum

Author

Xu, Chun ; Chai, Xiao-sen ; Zhang, Shu-lin ; Zhou, Jia

Author_Institution

Dept. of Microelectron., Fudan Univ., Shanghai, China

fYear

2010

Firstpage

1486

Lastpage

1488

Abstract

A specific and high-sensitive immunosensor for detecting pulmonary tuberculosis (TB) markers in human serum is presented by miniaturizing array of microelectrodes via micro electro-mechanical system (MEMS) for multi-channel electrochemical measurement. The immunosensor is consisted of six Au disk working electrodes, one Au counter electrode and one Ag/AgCl reference electrode. Semi-insulating poly(o-phenylenediamine)(PoPD) film was utilized to co-polymerize and immobilize antigen at the arrayed Au electrodes. The selective recognition of antibody to the corresponding antigen is quantitatively detected by the peak current in the presence of the redox probe, FcCH₂OH(FcM). Samples with antibody positive and antibody negative can be distinguished.

Keywords

biomedical electrodes; biosensors; gold; microelectrodes; microsensors; silver compounds; Ag-AgCl; Au; counter electrode; disk working electrodes; human serum; immunosensor; microelectromechanical system; multi-channel electrochemical measurement; pulmonary tuberculosis markers; redox probe; selective recognition; Arrays; Electrodes; Films; Gold; Humans; Immune system; Sensitivity;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State and Integrated Circuit Technology (ICSICT), 2010 10th IEEE International Conference on

Conference_Location

Shanghai

Print_ISBN

978-1-4244-5797-7

Type

conf

DOI

10.1109/ICSICT.2010.5667531

Filename

5667531