Title :
Transosseous application of low-intensity ultrasound at the tendon-bone interface affects the healing rate and up-regulates simultaneously the expression of collagen type I and tRNAGly
Author :
Papatheodorou, Loukia K. ; Grafanaki, Katerina ; Giannouli, Stamatina ; Fotiadis, Dimitrios I. ; Stathopoulos, Constantinos ; Malizos, Konstantinos N.
Author_Institution :
Dept. of Orthopaedic Surg. & Musculoskeletal Trauma, Univ. of Thessaly, Larissa
Abstract :
The present study investigates the effect of transosseous low-intensity pulsed ultrasound (LiUS) during lingamentization process on the healing at tendon graft-bone interface in rabbits. Analysis of the RT-PCR products showed statistically significant up-regulation of genes encoding collagen type I and tRNAGly in the study group compared to the control group. Histological examination indicated a faster healing rate and a more efficient lingamentization process after ultrasound treatment. Our results suggest that transosseous application of LiUS enhances the healing rate of the tendon graft-bone interface, possibly by affecting the expression levels of significant genes.
Keywords :
biological effects of acoustic radiation; bone; genetics; molecular biophysics; proteins; tissue engineering; ultrasonic effects; collagen type I gene expression; healing rate; lingamentization process; tRNAGly gene expression; tendon graft-bone interface; transosseous low-intensity pulsed ultrasound; Amino acids; Biochemistry; Biotechnology; Bones; Ligaments; Orthopedic surgery; Proteins; Rabbits; Tendons; Ultrasonic imaging;
Conference_Titel :
BioInformatics and BioEngineering, 2008. BIBE 2008. 8th IEEE International Conference on
Conference_Location :
Athens
Print_ISBN :
978-1-4244-2844-1
Electronic_ISBN :
978-1-4244-2845-8
DOI :
10.1109/BIBE.2008.4696755
