DocumentCode :
1641972
Title :
On the relationship between applied voltage and the duration of isoelectric focusing experiments in the development of a linear pH gradient
Author :
Johnson, J.F. ; Ouattara, S. ; Bohm, M.J. ; Gillock, E. ; Dawes, W.H. ; Dyer, R.A. ; Dyer, S.A. ; Hudson, W.B. ; Johnson, T.C. ; Consigli, R.A.
Author_Institution :
Dept. of Electr. & Comput. Eng., Kansas State Univ., Manhattan, KS, USA
Volume :
1
fYear :
1997
Firstpage :
527
Abstract :
The development of a linear pH gradient is necessary for the precise and consistent separation of biomolecules via isoelectric focusing techniques. Existing applications have used various voltage potentials in performing the separations, but the exact relationship between the voltage and the required time duration on the development of a linear pH gradient has not been determined. Certain applications, such as separations within the microgravity environment, can benefit from the use of low voltages, whereas other applications are not constrained by voltage limitations. In general, as the voltage increases, the time duration required for high-definition separation appears to be reduced, but the effect of these changes on the linearity of the pH gradient was not previously known. A mixture of ampholytes with a pH range of 3 to 10 was used in the investigation of this relationship, and voltage levels from 125 V to 1000 V were employed. A 6% polyacrylamide gel in a cylindrical configuration was used as the medium in all the experiments. The pH profiles of the gels were determined immediately after the conclusion of each time-duration experiment, without disturbing the integrity of the gel. This was accomplished by using a specialized iridium-oxide pH sensor that can be placed directly on the gel surface. The authors present their findings on the ability to produce linear pH gradients as a function of the voltage level applied and the time duration of the isoelectric focusing experiment
Keywords :
bioelectric phenomena; biological techniques; gels; molecular biophysics; 125 to 1000 V; ampholytes mixture; biomolecules separation; cylindrical configuration; isoelectric focusing experiment; linear pH gradient development; microgravity environment; pH gradient; polyacrylamide gel; specialized iridium-oxide pH sensor; voltage limitations; Biology computing; Ear; Electrodes; Fractionation; Gravity; Linearity; Low voltage; Molecular biophysics; Runtime; Shape;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Instrumentation and Measurement Technology Conference, 1997. IMTC/97. Proceedings. Sensing, Processing, Networking., IEEE
Conference_Location :
Ottawa, Ont.
ISSN :
1091-5281
Print_ISBN :
0-7803-3747-6
Type :
conf
DOI :
10.1109/IMTC.1997.604005
Filename :
604005
Link To Document :
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