Title :
Multiphoton multifoci modulation microscopy for high-speed fluorescence lifetime imaging
Author :
Straub, Adam A. ; Howard, Scott S. ; Xu, Chris
Author_Institution :
Sch. of Appl. & Eng. Phys., Cornell Univ., Ithaca, NY, USA
Abstract :
We demonstrate a novel depth-resolved parallel-acquisition fluorescence lifetime imaging technique, and present in-vivo images of mouse brain. Analysis shows up to two orders-of-magnitude speed improvement over standard serial-acquisition techniques.
Keywords :
biomedical optical imaging; brain; fluorescence; high-speed optical techniques; image reconstruction; medical image processing; multiphoton processes; optical microscopy; radiative lifetimes; depth-resolved parallel-acquisition fluorescence lifetime imaging technique; high-speed fluorescence lifetime imaging; image reconstruction; in-vivo mouse brain images; multiphoton multifoci modulation microscopy; standard serial-acquisition techniques; Fluorescence; Frequency measurement; Frequency modulation; Microscopy; Standards;
Conference_Titel :
Lasers and Electro-Optics (CLEO), 2012 Conference on
Conference_Location :
San Jose, CA
Print_ISBN :
978-1-4673-1839-6
