Title :
Synthesis of Lactosylated Piperazinyl Porphyrin and its Interaction with DNA
Author :
Li, Heping ; Wang, Zhoudong
Author_Institution :
Coll. of Chem. & Environ. Eng., Changsha Univ. of Sci. & Technol., Changsha
Abstract :
A water-soluble lactosylated piperazinyl porphyrin, 5- mono[4-(4´-lactobionylaminopiperazinyl)- phenyl]-10,15,20- triphenyl-porphyrin (MLPP), was synthesized basing on the porphyrin´s special affinity for cancer cells and the antitumor activity of piperazine compounds. Its structure was characterized by UV-Vis spectra, elemental analysis and 1H NMR. A model for the interaction between MLPP and DNA was built, and the binding mechanism between MLPP and cell molecules was investigated by UV-Vis spectra and fluorescence spectrum.
Keywords :
DNA; biochemistry; cancer; cellular biophysics; fluorescence; molecular biophysics; nuclear magnetic resonance; tumours; ultraviolet spectra; visible spectra; 1H NMR; DNA interactions; UV-Vis spectra; antitumor activity; binding mechanism; cancer cells; cell molecules; elemental analysis; fluorescence spectrum; water-soluble lactosylated piperazinyl porphyrin synthesis; Cancer; Chemical analysis; Chemical elements; Chemical technology; Chemistry; DNA; Educational institutions; Fluorescence; Nuclear magnetic resonance; Spectroscopy;
Conference_Titel :
Bioinformatics and Biomedical Engineering, 2008. ICBBE 2008. The 2nd International Conference on
Conference_Location :
Shanghai
Print_ISBN :
978-1-4244-1747-6
Electronic_ISBN :
978-1-4244-1748-3
DOI :
10.1109/ICBBE.2008.34
