Isoliquiritigen Down-Regulating the Expression of the Matrix Metalloproteinases-2 in Human Fibrosarcoma HT1080 Cell

Isoliquiritigenin (ISL ), a natural pigment wi th the simple chalconestructure 4,2 V,4V-trihydroxychalcone, has been well characterized to possess the pleiotropic anti-cancer capabilities in several cancer cell lines. This Study is to investigate the effects of isoliquiritigenin on the invasion in vitro and the expression of matrix metalloproteinase-2 (MMP-2) in human fibrosarcoma HT1080 cells in absence of cytotoxicity. According to our results, it showed that isoliquiritigenin exerted a concentration-dependent inhibitory effect on the proliferation of HT1080 cells. Treatment with isoliquiritigenin could significantly decline the invasive ability of HT1080 cells compared to the control cells (P<0.01) in the in vitro invasion assay. Further analysis with gelatin zymography and western blotting revealed that both the activity and the expression of MMP-2 decreased dramatically in a concentration-dependent manner (P<0.01). Synthetically, these results indicated that isoliquiritigenin could significantly inhibit the invasiveness of HT1080 cells in vitro; Meanwhile, this effects could be mainly carried out by down-regulating the expression of MMP-2. Isoliquiritigenin could be used for a potential therapeutic agent in prevention and treatment of cancer.