Heterologous Expression of Bovine Lactoferricin in Escherichia Coli

Author

Wang, HaiKuan ; Zhao, XinHuai ; Liu, RuiJuan ; Wei, Qi ; Lu, Fuping

Author_Institution

Tianjin Key Lab. of Ind. Microbiol., Tianjin Univ. of Sci. & Technol., Tianjin

fYear

2008

Firstpage

395

Lastpage

398

Abstract

According to the bias of codon utilization of Escherichia coli, a fragment encoding bovine lactoferricin(LfcinB) has been cloned and expressed in the Escherichia coli under the control of the T7 promoter. The target peptide was expressed by IPTG induction, purified by affinity chromatography with 6XHis tagged resin. The recombinant bovine lactoferricin appears to be successfully expressed, as it displays antibacterial activity (antibacterial assay). Moreover, the identity of the recombinant product was estimated by Tricine-SDS-PAGE.

Keywords

genetics; microorganisms; molecular biophysics; 6XHis tagged resin; Escherichia coli; IPTG induction; LfcinB; T7 promoter; Tricine-SDS-PAGE; affinity chromatography; antibacterial activity; bovine lactoferricin bias; bovine lactoferricin heterologous expression; fragment encoding bovine lactoferricin; recombinant bovine lactoferricin; Anti-bacterial; Bovine; Capacitive sensors; DNA; Encoding; Peptides; Production; Proteins; Purification; Sequences;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioinformatics and Biomedical Engineering, 2008. ICBBE 2008. The 2nd International Conference on

Conference_Location

Shanghai

Print_ISBN

978-1-4244-1747-6

Electronic_ISBN

978-1-4244-1748-3

Type

conf

DOI

10.1109/ICBBE.2008.100

Filename

4534980