Title :
Heterologous Expression of Bovine Lactoferricin in Escherichia Coli
Author :
Wang, HaiKuan ; Zhao, XinHuai ; Liu, RuiJuan ; Wei, Qi ; Lu, Fuping
Author_Institution :
Tianjin Key Lab. of Ind. Microbiol., Tianjin Univ. of Sci. & Technol., Tianjin
Abstract :
According to the bias of codon utilization of Escherichia coli, a fragment encoding bovine lactoferricin(LfcinB) has been cloned and expressed in the Escherichia coli under the control of the T7 promoter. The target peptide was expressed by IPTG induction, purified by affinity chromatography with 6XHis tagged resin. The recombinant bovine lactoferricin appears to be successfully expressed, as it displays antibacterial activity (antibacterial assay). Moreover, the identity of the recombinant product was estimated by Tricine-SDS-PAGE.
Keywords :
genetics; microorganisms; molecular biophysics; 6XHis tagged resin; Escherichia coli; IPTG induction; LfcinB; T7 promoter; Tricine-SDS-PAGE; affinity chromatography; antibacterial activity; bovine lactoferricin bias; bovine lactoferricin heterologous expression; fragment encoding bovine lactoferricin; recombinant bovine lactoferricin; Anti-bacterial; Bovine; Capacitive sensors; DNA; Encoding; Peptides; Production; Proteins; Purification; Sequences;
Conference_Titel :
Bioinformatics and Biomedical Engineering, 2008. ICBBE 2008. The 2nd International Conference on
Conference_Location :
Shanghai
Print_ISBN :
978-1-4244-1747-6
Electronic_ISBN :
978-1-4244-1748-3
DOI :
10.1109/ICBBE.2008.100
