Title :
Cloning of Trehalose-6-Phosphate Phosphatase and Transformation to Tobacco
Author :
Bei, Guo ; Jiang Xiang-ning
Author_Institution :
Dept. of Biotechnol., Beijing Univ. of Agric., Beijing
Abstract :
The trehalose-6-phosphate phosphatase (TPP) is one of enzymes related to trehalose biosynthesis. But the researches of TPP were mainly focused in microorganism; few in the plant were reported. In this study, the TPP gene was cloned from Arabidopsis seedling by using RT-PCR (reverse transcription PCR) method. Then it was constructed to pET30a (+) vector and overexpressed in E.coli. Subsequently, the TPP was ligated into the plant expressional vector pBI121 and transformed to tobacco. Three transgenic individuals have obtained and one of them has transcribed successfully through Real-time QPCR detection. The trehalose content has less visible increase in P3 individual, but there was no obvious difference between the transgenic plant and control under salt stress.
Keywords :
biological techniques; biomechanics; cellular biophysics; enzymes; microorganisms; molecular biophysics; Arabidopsis seedling; E.coli overexpression; cloning; enzymes; microorganism; pET30a vector; plant expressional vector pBI121; real-time QPCR detection; reverse transcription PCR method; salt stress; tobacco; transgenic plant; trehalose biosynthesis; trehalose-6-phosphate phosphatase; Agriculture; Biochemistry; Biotechnology; Cloning; Educational institutions; Encoding; Ethanol; Fungi; Microorganisms; Stress;
Conference_Titel :
Bioinformatics and Biomedical Engineering, 2008. ICBBE 2008. The 2nd International Conference on
Conference_Location :
Shanghai
Print_ISBN :
978-1-4244-1747-6
Electronic_ISBN :
978-1-4244-1748-3
DOI :
10.1109/ICBBE.2008.108
