Analysis on the Microbial Diversity of Qinghai High Salt Content Oil Reservoirs

Author

Yuehui, She ; Beiwen, Zheng ; Fan, Zhang ; Wang Zhengliang ; Fuchang, Shu ; Lingge, Zhou ; Xiang Tingsheng ; Longjiang, Yu

Author_Institution

Coll. of Biol. Sci. & Technol., Huazhong Univ. of Sci. & Technol., Wuhan

fYear

2008

Firstpage

780

Lastpage

783

Abstract

Cultivation method and sequencing analysis of 16S rRNA genes were applied to analyze the microbial diversity of Qinghai oil reservoirs. The results of culture demonstrated that there existed a phylogenetically diverse and complex microbial community in Qinghai oil field formation. 10% randomly selected clones were analyzed by DNA sequencing. Sequencing of representative clones of each phylotype showed that the community structure was mainly constituted by Marinobacterium and Psudomonas, and there were some kinds of uncultured microbe existed in the sample. The results of this study provide further insight into the composition of microbial communities of high salt content oil reservoirs.

Keywords

DNA; biological techniques; environmental factors; genetics; hydrocarbon reservoirs; microorganisms; molecular biophysics; DNA sequencing analysis; Marinobacterium; Psudomonas; Qinghai high salt content oil reservoirs; cultivation method; oil reservoir microbial diversity; rRNA genes; Biochemical analysis; Chemical technology; Cloning; Cultural differences; Educational institutions; Hydrocarbon reservoirs; Microorganisms; Petroleum; Phylogeny; Temperature;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioinformatics and Biomedical Engineering, 2008. ICBBE 2008. The 2nd International Conference on

Conference_Location

Shanghai

Print_ISBN

978-1-4244-1747-6

Electronic_ISBN

978-1-4244-1748-3

Type

conf

DOI

10.1109/ICBBE.2008.190

Filename

4535070