DocumentCode :
1655507
Title :
Construction and investigation of a DNA sensing system based on a magnetic molecular beacon probe
Author :
Xu, Shichao ; Yang, Juan ; Zhang, Jimci ; Dai, Zhao ; Sun, Bo ; Feng, Tielin ; Zi, Yan
Author_Institution :
Sch. of Environ. & Chem. Eng., Univ. Tianjin, Tianjin, China
fYear :
2010
Firstpage :
771
Lastpage :
772
Abstract :
We presented a fast, specific, and sensitive DNA sensing system, which composed of a CdTe/Fe3O4 magnetic core-shell quantum dots (energy donor), a commercial quencher (BHQ2; energy acceptor), and a designed single strand Toxoplasma gondii DNA. The designed single strand Toxoplasma gondii DNA was applied to link the energy donor and acceptor, and target DNA was detected based on mechanism of fluorescence resonance energy transfer. The CdTe quantum dots, Fe3O4 magnetic nanoparticles, CdTe/Fe3O4magnetic core-shell quantum dots, and sensing probe were step-wisely prepared. Properties of synthesized quantum dots were investigated by transmission electron microscopy and fluorescence spectrum, respectively. Specificity and sensitivity of sensing probe was determined by measuring the recovery of fluorescence intensity. The resulting data revealed the sensing system was successfully fabricated, and which has relative high sensitivity and specificity. Moreover, a simple modeling of the current sensing system/probe was primarily created, and which was utilized to theoretically illustrate and quantitatively investigate some surface properties of the sensing probe. We expect the current modeling can profit the current research and promote the sensitivity and specificity of the sensing system.
Keywords :
DNA; bioluminescence; biosensors; cadmium compounds; iron compounds; magnetic materials; quantum dots; transmission electron microscopy; CdTe-Fe3O4; DNA sensing system; energy donor; fluorescence resonance energy transfer; fluorescence spectrum; magnetic core-shell quantum dots; magnetic molecular beacon probe; magnetic nanoparticles; single strand Toxoplasma gondii DNA; transmission electron microscopy; DNA; Energy exchange; Fluorescence; Iron; Magnetic cores; Magnetic resonance; Nanoparticles; Probes; Quantum dots; Sensitivity and specificity;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Nanoelectronics Conference (INEC), 2010 3rd International
Conference_Location :
Hong Kong
Print_ISBN :
978-1-4244-3543-2
Electronic_ISBN :
978-1-4244-3544-9
Type :
conf
DOI :
10.1109/INEC.2010.5424464
Filename :
5424464
Link To Document :
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