Purification of virus particles by ceramic hydroxyapatite chromatography on microfluidic chip

This paper presents a microfluidic chip to purify virus from the particle laden solution like body fluid. Because many of virus infectious diseases occur in the developing region, more compact and low-cost device for virus detection from the patients´ body fluid is required. We proposed a microfluidic chip for virus isolation by ceramic hydroxyapatite chromatography. This microfluidic chip utilized a column for on-chip chromatography. Ceramic hydroxyapatite particles were packed in the column and viruses and impurities were adsorbed on the hydroxyapatite particles. We have succeeded in purification virus particle by buffer displacement. Furthermore PNA immobilized on the glass can detect the targeted virus genome so that in situ virus detection would be possible by anybody, anywhere, anytime.