Screening and Phylogenetic Analysis of Halophilic Bacteria from the Deep Sea Water at Low Temperature

Author

Ye Shuhong ; Li Yuanguang ; Wan Huiping ; He Lianfang ; Wang Jihui ; CaoFang

Author_Institution

Bioeng. Coll., East China Univ. of Sci. & Technol., Shanghai

fYear

2008

Firstpage

1050

Lastpage

1053

Abstract

Three strains of halophilic bacteria were screened from the deep sea water at low temperature. Their phylogenetic positions on the basis of amplification, comparison and analysis of almost complete 16S rRNA gene were cloned and determined by neighboring-joining analyses. Phylogenetic analysis indicated that two strains belong to Pseudomonas.sp. The third stain was classified as genus Acinetobacter .sp. The phylogenetic tree was produced from 16S rRNA of 3 halophilic strains and other 30 strains using 16S rRNA gene as out-group. Two strains (B2 and CY11) were clustered with many Pseudomonas.sp strains. One strain (LT4) was clustered with Acinetobacter baumannii.

Keywords

genetics; microorganisms; molecular biophysics; oceanography; organic compounds; 16S rRNA gene; Acinetobacter baumannii; Pseudomonas sp; deep sea water; halophilic bacteria; neighboring-joining analysis; phylogenetic analysis; phylogenetic tree; screening; Biotechnology; Capacitive sensors; Chemical technology; Food technology; Marine technology; Microorganisms; Ocean temperature; Phylogeny; Psychology; Sequences;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioinformatics and Biomedical Engineering, 2008. ICBBE 2008. The 2nd International Conference on

Conference_Location

Shanghai

Print_ISBN

978-1-4244-1747-6

Electronic_ISBN

978-1-4244-1748-3

Type

conf

DOI

10.1109/ICBBE.2008.257

Filename

4535137