DocumentCode :
1656485
Title :
Manipulation of primary cilia using optical tweezers
Author :
Harada, Yoshinori ; Ota, Taisuke ; Takamatsu, Tetsuro
Author_Institution :
Dept. of Pathology & Cell Regulations, Kyoto Prefectural Univ. of Medicine
fYear :
2006
Firstpage :
1
Lastpage :
4
Abstract :
Nonmotile primary cilia are expressed in most renal epithelial cells. The primary cilia of renal epithelia bend with the urinary flow within the renal tubules. It has been postulated that the cilia are a mechano-sensor of urinary flow. However, the mechanical characteristics of an individual primary cilium are not fully understood. In the present study, we successfully manipulated micron-sized primary cilia of the Madin-Darby canine kidney (MDCK) cell lines derived from the collecting duct, by using optical tweezers. The primary cilia of MDCK cells, cultured on flexible plastic films, could be clearly visualized from the side by video microscopy. Optical tweezers utilize the radiation pressure from the concentrated laser beam to trap and manipulate a small microparticle, and forces on the objects in the trap can be evaluated. We showed that we could apply forces in the piconewton range to a polystyrene bead fixed on the tip of the primary cilium by using optical tweezers; by that means, the primary cilium could be bent and stretched. The elasticity of each primary cilium was analyzed by measuring the displacement of the bead
Keywords :
biomechanics; biosensors; cell motility; cellular effects of radiation; elasticity; kidney; laser applications in medicine; radiation pressure; Madin-Darby canine kidney cell lines; collecting duct; elasticity; laser beam; manipulation; mechano-sensor; nonmotile primary cilia; optical tweezers; piconewton range; plastic films; polystyrene bead; radiation pressure; renal epithelial cells; renal tubules; urinary flow; video microscopy; Cells (biology); Charge carrier processes; Displacement measurement; Ducts; Elasticity; Laser beams; Microscopy; Optical films; Plastic films; Visualization;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Micro-NanoMechatronics and Human Science, 2006 International Symposium on
Conference_Location :
Nagoya
Print_ISBN :
1-4244-0717-6
Electronic_ISBN :
1-4244-0718-1
Type :
conf
DOI :
10.1109/MHS.2006.320293
Filename :
4110396
Link To Document :
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