Title :
Development of a microfluidic perfusion system for patch clamp recording
Author :
Runhuai Yang ; Lai, King W. C. ; Ning Xi ; Jie Yang
Author_Institution :
Dept. of Mech. & Biomed. Eng., City Univ. of Hong Kong, Hong Kong, China
Abstract :
In this paper, the design of a microfluidic perfusion system for patch clamp recording is reported. The microfluidic perfusion system mainly consists of a polydimethylsiloxane (PDMS) chamber and a micro drug delivery pipette. The performance of the perfusion system was verified by conducting patch clamp recording of neuroblastoma cells. This system provides a method to precisely perform drug delivery without any disturbance of the seal condition.
Keywords :
bioMEMS; cellular transport; drug delivery systems; microfluidics; polymers; PDMS; microdrug delivery pipette; microfluidic perfusion system design; neuroblastoma cells; patch clamp recording; perfusion system performance; polydimethylsiloxane chamber; seal condition; Clamps; Drug delivery; Drugs; Extracellular; Glass; Microfluidics; Seals;
Conference_Titel :
Nanotechnology (IEEE-NANO), 2014 IEEE 14th International Conference on
Conference_Location :
Toronto, ON
DOI :
10.1109/NANO.2014.6968092
