Title :
Fourier analysis of digital STEM images of rat lens in the study of transparency
Author :
Vaezy, Shahram ; Clark, John I. ; Gisselberg, Margo ; Cantino, Marie E.
Author_Institution :
Washington Univ., Seattle, WA, USA
Abstract :
Fourier components of the microstructure of the rat lens of varying turbidity were measured for the range 100-1100 nm from scanning transmission electron microscopy (STEM) images. The results indicate that the spectral energy content of these images decreases in the 100-200-nm range, stays approximately constant in the 200-300-nm range, and increases in the 300-1100-nm range, as one moves from a transparent area of the lens to an opaque area. A total increase of 19% in the spectral energy content for the 200-1100 nm range accounts for the opacity
Keywords :
Fourier analysis; eye; lenses; scanning-transmission electron microscope examination of materials; transparency; digital STEM images; eye; image spectral energy content; lens microstructure; opaque area; rat lens; scanning transmission electron microscopy; transparent area; turbidity; Area measurement; Biomedical engineering; Electrons; Energy measurement; Fluctuations; Image analysis; Lenses; Microstructure; Proteins; X-ray scattering;
Conference_Titel :
Engineering in Medicine and Biology Society, 1989. Images of the Twenty-First Century., Proceedings of the Annual International Conference of the IEEE Engineering in
Conference_Location :
Seattle, WA
DOI :
10.1109/IEMBS.1989.95775
