Isolation and Identification of Two High Effective Atrazine Degrading Microbe

Collecting the soil which had applied Atrazine for more then 5 years from The Xiang Fang experimental farm of HarBin and the corn field of WuChang in Heilongjiang Province. We isolated 7 microbes which have the ability of Atrazine degrading by the way of the traditional enrichment and isolation of bacteria in the laboratory, then we filtrated 2 microbes which the ability of atrazine degrading was relatively higher and named J₂ and J₆. They were inoculated into 20 mL the corresponding medium which the concentration of Atrazine was 100 mg/L by 5% percent of the medium, they could degradated the Atrazine completely in 5 days and 4 days respectively. According to the condition of enrichment and isolation, we known that J₂ used Atrazine as the sole nitrogen source and J₆ used Atrazine as the sole carbon and nitrogen source. The preliminary identifications indicated that J₂ belonged to Microbacterium sp and J₆ belonged to Aureobacterium.