Effect of Montmorillonite on Diffusion of Creatinine Between Blood Vessel and Intestine

To study the effect of montmorillonite promoting diffuse of creatinine from blood vessel to intestine and preventing absorption of creatinine in intestinal perfusate. Methods The intestine tract and blood vessel of rats were perfused circularly with intestinal perfusate or blood vessel perfusate containing creatinine 80 mg/L, respectively. The concentration of creatinine was determined by basic picric acid method. Results When the blood vessel and intestinal tract were perfused circularly by vascular perfusate containing creatinine and intestine perfusate without creatinine, respectively, the concentration of creatinine in vascular perfusate decreased little by little, and the concentration of creatinine in intestine perfusate increased slowly. The concentration of creatinine of intestine and blood vessel perfusate was decreased in montmorillonite of 1.0 g/kg and 0.5 g/kg groups compared with control (P<0.01). The blood vessel and intestinal tract were perfused circularly by vascular perfusate without creatinine and intestine perfusate with creatinine, respectively, the concentration of creatinine in intestine perfusate decreased little by little, and the concentration of creatinine in vascular perfusate increased slowly. When montmorillonite was administrated into the intestinal tract, the concentration of creatinine in intestine and blood vessel perfusate of montmorillonite of 1.0 g/kg and 0.5 g/kg group was decreased obviously (P<0.01). Conclusion Creatinine can diffuse from blood vessel to intestine and is reabsorbed in intestine. Montmorillonite can promote the diffuse of creatinine to intestine tract and can decrease absorption of creatinine in intestinal tract.