Title :
Thermal Gradient Instrument for Gene Amplification
Author_Institution :
Sch. of Comput. Eng., Qingdao Technol. Univ., Qingdao
Abstract :
This paper presents an integrated thermal gradient device for gene amplification. This instrument consists of a 16- bit MCU chip with 256k flash-ROM, six thermoelectric heat pumps placed on top of a micro-machined silicon chip integrated with three temperature sensors, three power amplifiers, three integrated 12-bit analog-to-digitals, three integrated 12-bit digital -to- analogs and a lid-heater. It is a new gene amplification technique that is characterized by periodic temperature ramping. There are three channels that are determined by temperature control system individually in the instrument. The advantages of this system are the high stability of the temperature, rapid thermo-cycling and the easy of establishment of the adequate conditions for the nested polymerase chain reaction.
Keywords :
biochemistry; biomedical equipment; genetics; MCU chip; flash ROM; gene amplification; integrated analog to digital covertors; integrated digital to analogs covertors; integrated thermal gradient device; lid heater; micromachined silicon chip; nested polymerase chain reaction; periodic temperature ramping; power amplifiers; rapid thermocycling; storage capacity 12 bit; temperature sensors; thermal gradient instrument; thermoelectric heat pump; Analog-digital conversion; DNA; Instruments; Laboratories; Polymers; Sampling methods; Signal sampling; Temperature control; Temperature sensors; Thermal engineering;
Conference_Titel :
Bioinformatics and Biomedical Engineering, 2008. ICBBE 2008. The 2nd International Conference on
Conference_Location :
Shanghai
Print_ISBN :
978-1-4244-1747-6
Electronic_ISBN :
978-1-4244-1748-3
DOI :
10.1109/ICBBE.2008.697
