Nanoprobe insertion for nanoinjection based on E-SEM nanorobotic manipulation

This paper presents a nanoprobe insertion for nanoinjection applications for biological species based on environmental-scanning electron microscope (E-SEM) nanorobotic manipulation system. Based on the bio-nano manipulation system, we have been proposed a nano-injection method by nanoprobe insertion. The nanoprobe is designed to have a sharp tip to insert the probe into biological specimen. It is fabricated by focused ion beam (FIB) process. The nano-scale size is important for minimal damage and enough rigidity to biological specimen. A Caenorhabditis elegans (C. elegans) is used as a target biological specimen, which is one of the important model organism for diseases analysis. The direct nanoinjection technique into a biological organism is needed to transport fluorescent materials or specific biological proteins into its specific cell as in-vivo experiment. The proposed system is considered to be important as future nano-surgery system for life innovation using model organism.