Title :
Novel integration method for chemical and enzyme sensor array by using microcontact printing
Author :
Suzuki, Masayasu ; Nomura, Akitaka ; Yamamoto, Mizuki ; Minakuchi, Kenta ; Iribe, Yasunori
Author_Institution :
Dept. of Electr. & Electron. Eng., Univ. of Toyama, Toyama, Japan
Abstract :
The fluorescence-based integrated chemical and enzyme sensor arrays on which two kinds of 10 μm i.d. sensor spots were arranged alternately on one chip, were developed by using microcontact printing. FITC, ruthenium complex and europium tetracycline was stamped onto a DLC sputtered glass slides for pH, oxygen and hydrogen peroxide sensors, respectively. After printing of oxygen sensor spots, pH sensor spots were stamped between oxygen sensor spots by using the same PDMS stamp. In the same way, glucose oxidase and lactate oxidase spots were stamped alternately onto H2O2 sensor spots. Each sensors in the integrated sensors showed specific responses independently.
Keywords :
chemical sensors; enzymes; sensor arrays; soft lithography; enzyme sensor array; europium tetracycline; fluorescence-based integrated chemical; glucose oxidase; hydrogen peroxide sensor; integration method; lactate oxidase spot; microcontact printing; oxygen sensor spot; pH sensor spot; ruthenium complex; sputtered glass slide; Arrays; Biochemistry; Fluorescence; Ink; Sensors; Sugar;
Conference_Titel :
Micro-NanoMechatronics and Human Science (MHS), 2010 International Symposium on
Conference_Location :
Nagoya
Print_ISBN :
978-1-4244-7995-5
DOI :
10.1109/MHS.2010.5669540
