Title :
Automatic measurement system for biological applications based on pH using ISFET sensor probe
Author :
Yamada, Akira ; Mohri, Satoshi ; Nakamura, Michihiro ; Naruse, Keiji
Author_Institution :
Dept. of Mech. Syst. Eng., Hiroshima Inst. of Technol., Hiroshima, Japan
Abstract :
Throughput in the cell response analysis is expected to progress in medicine and biology. In this paper, we construct a method for the measurement of cell activity that is measured by the pH fluctuation of the solution around the cell. To improve throughput of the measurements, first we constructed an automatic pH measurement system using ion-sensitive field-effect transistor (ISFET), and the system composed of 96-well microplate, pump, XYZ stage, and PC. The basic data of the cell activity was taken from human embryonic kidney 293 (HEK 293) cells by the use of flow-through type measuring chamber.
Keywords :
biochemistry; biomedical measurement; cellular biophysics; chemical sensors; fluctuations; ion sensitive field effect transistors; kidney; pH measurement; pumps; ISFET sensor probe; automatic measurement system; automatic pH measurement system; biological applications; cell activity; cell response analysis; flow-through type measuring chamber; human embryonic kidney cells; ion-sensitive field-effect transistor; microplate; pH fluctuation; pump; Q measurement; Switches;
Conference_Titel :
Micro-NanoMechatronics and Human Science (MHS), 2010 International Symposium on
Conference_Location :
Nagoya
Print_ISBN :
978-1-4244-7995-5
DOI :
10.1109/MHS.2010.5669583
