Automatic measurement system for biological applications based on pH using ISFET sensor probe

Author

Yamada, Akira ; Mohri, Satoshi ; Nakamura, Michihiro ; Naruse, Keiji

Author_Institution

Dept. of Mech. Syst. Eng., Hiroshima Inst. of Technol., Hiroshima, Japan

fYear

2010

Firstpage

19

Lastpage

24

Abstract

Throughput in the cell response analysis is expected to progress in medicine and biology. In this paper, we construct a method for the measurement of cell activity that is measured by the pH fluctuation of the solution around the cell. To improve throughput of the measurements, first we constructed an automatic pH measurement system using ion-sensitive field-effect transistor (ISFET), and the system composed of 96-well microplate, pump, XYZ stage, and PC. The basic data of the cell activity was taken from human embryonic kidney 293 (HEK 293) cells by the use of flow-through type measuring chamber.

Keywords

biochemistry; biomedical measurement; cellular biophysics; chemical sensors; fluctuations; ion sensitive field effect transistors; kidney; pH measurement; pumps; ISFET sensor probe; automatic measurement system; automatic pH measurement system; biological applications; cell activity; cell response analysis; flow-through type measuring chamber; human embryonic kidney cells; ion-sensitive field-effect transistor; microplate; pH fluctuation; pump; Q measurement; Switches;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro-NanoMechatronics and Human Science (MHS), 2010 International Symposium on

Conference_Location

Nagoya

Print_ISBN

978-1-4244-7995-5

Type

conf

DOI

10.1109/MHS.2010.5669583

Filename

5669583