Title :
Microfluidic switch for embryo and cell sorting
Author :
Chen, C.C. ; Zappe, S. ; Sahin, O. ; Zhang, X.J. ; Furlong, E.E.M. ; Fish, M. ; Scott, M. ; Solgaard, O.
Author_Institution :
Stanford Univ., CA, USA
Abstract :
We present measurements and simulation of fluid flow and sphere motion in a pressure-driven microfluidic sorter designed for Drosophila embryos. We simulated the flow fields for different channel layouts and flow parameters to find geometries and conditions for optimised embryo movement. The microfluidic switch has a designed switching time of 360 /spl mu/s. We validated the sorter design with fluid experiments in devices fabricated from silicon and Pyrex wafers. The experimental and simulated results are compared both qualitatively and quantitatively by examining the concentration distribution of fluids. This type of microfluidic sorter is promising for micromanipulation of a variety of biological cells, and provides automated operations for higher throughput and accuracy.
Keywords :
cellular transport; elemental semiconductors; flow simulation; glass; microfluidics; micromanipulators; microswitches; semiconductor device models; silicon; zoology; 360 mus; Si; biological cells; cell sorting; drosophila embryos; embryo; embryo movement; flow fields; flow parameters; fluid flow simulation; fluids concentration distribution; microfluidic sorter; microfluidic switch; micromanipulation; pressure-driven microfluidic sorter; pyrex wafers; silicon; Embryo; Fluid flow; Fluid flow measurement; Geometry; Microfluidics; Motion measurement; Silicon; Solid modeling; Sorting; Switches;
Conference_Titel :
TRANSDUCERS, Solid-State Sensors, Actuators and Microsystems, 12th International Conference on, 2003
Conference_Location :
Boston, MA, USA
Print_ISBN :
0-7803-7731-1
DOI :
10.1109/SENSOR.2003.1215559
