Alginate strings with genetically engineered fibroblasts and their application in spinal cord regeneration

We have optimized a method for producing strings of alginate bioconjugated with a laminin pentapeptide that aids in cell adhesion. These strings are 400-500 μm in thickness and could be bundled to act as a graft at the site of injury. In vitro studies have shown that rat (NB2a) and human (SHSY5Y) neuroblastoma cell lines adhere to, and differentiate on these modified alginate strings. The strings also have the capacity to hold genetically modified fibroblasts that release neurotrophins that would aid in neuronal regeneration. These strings are strong enough to be surgically transplanted to the spinal cord, and may ultimately help in bridging the gap at the injury site.