Title :
Screening a novel high effective dioscorea zingiberensis degradation strain
Author :
Lu, Yanjun ; Guo, Jifeng
Author_Institution :
Sch. of Civil Eng., Xi´an Technol. Univ., Xi´an, China
Abstract :
12 strains of cellulolytic microorganism were isolated from the AB reactor of dioscorea zingiberensis wastewater treatment. A bacterial strain was obtained through the screening experiments of decomposition of dioscorea zingiberensis, which had high degradability for dioscorea zingiberensis and was named D-1. The gene sequence obtained from the DNA extraction and polymerase chain reaction (PCR) amplification was compared with NCBI Genbank of U.S.A., and the strain was preliminary identified to be Clostridium cellulosolvens. The optimum growth conditions were pH 5.0, 30°C, 100 r/min.
Keywords :
crops; microorganisms; wastewater treatment; water pollution; AB reactor; Clostridium cellulosolvens; D-1; DNA extraction; PCR amplification; bacterial strain; cellulolytic microorganism; decomposition; dioscorea zingiberensis degradation strain; gene sequence; polymerase chain reaction; temperature 30 C; wastewater treatment; Clostridium cellulosolvens; dioscorea zingiberensis degradation strain; polymerase chain reaction (PCR); screening;
Conference_Titel :
Water Resource and Environmental Protection (ISWREP), 2011 International Symposium on
Conference_Location :
Xi´an
Print_ISBN :
978-1-61284-339-1
DOI :
10.1109/ISWREP.2011.5893294