Alginate strings acting as a directional gradient in spinal cord regeneration

We are designing a spinal cord graft that would aid in directionally specific neuronal regeneration. We plan to use genetically engineered cells that release neurotrophic factors ensheathed in modified alginate strings. Thus far, we have a method for making strings of alginate bioconjugated with a laminin pentapeptide (YIGSR), which support cell adhesion. These strings are 400-500 μm in thickness. In vitro studies have shown that rat (NB2a) and human (SHSY5Y) neuroblastoma cell lines adhere to, and differentiate on these modified alginate strings. Currently we are aiming to establish a growth factor gradient to promote neuronal differentiation in a desired direction. These modified alginate strings, when surgically transplanted in the spinal cord, would set up a physical gradient providing directional regeneration of the adherent neuronal cells.