Oligonucleotide structure affects behavior of oligoplexes

Author

Sundaram, Suresh ; Roth, Charles M.

Author_Institution

Dept. of Chem. & Biochem. Eng., Rutgers Univ., Piscataway, NJ, USA

fYear

2003

Firstpage

317

Lastpage

318

Abstract

The objective of this work is to investigate the effect of oligonucleotide structure on the formation and biological properties of oligonucleotide/poly-L-lysine (ODN/PLL) complexes, or "oligoplexes". Two structurally different ODN sequences RBG (rabbit β globin) 33 and RBG147 were used in the study. The difference in ODN binding and hence complex formation was analyzed by a gel retardation assay. In addition, complex formation in buffer versus in serum-free medium was studied. The oligoplexes also displayed different cellular uptake efficiencies. Differences in the biological activity were attributed to the varying characteristics of the oligoplexes due to ODN structural difference.

Keywords

biomembrane transport; macromolecules; molecular biophysics; molecular configurations; RBG147; antisense; biological activity differences; buffer; cellular uptake; oligoplexes; poly-L-lysine; rabbit β globin; serum-free medium; structural difference; Amino acids; Biomedical engineering; Chemical engineering; Fluorescence; Microscopy; Phase locked loops; RNA; Rabbits; Sequences; Stability;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioengineering Conference, 2003 IEEE 29th Annual, Proceedings of

Print_ISBN

0-7803-7767-2

Type

conf

DOI

10.1109/NEBC.2003.1216123

Filename

1216123