Quantitative optical biomarkers for non-invasive detection of cancerous transformation in live, 3D squamous epithelia

Author

Pouli, Dimitra ; Varone, Antonio ; Xylas, Joanna ; Quinn, Kyle P. ; Hunter, Michael ; Georgakoudi, Irene ; McLaughlin-Drubin, Margaret E. ; Munger, Karl

Author_Institution

Dept. of Biomed. Eng., Tufts Univ., Medford, MA, USA

fYear

2014

fDate

25-27 April 2014

Firstpage

1

Lastpage

2

Abstract

Automated image analysis algorithms applied to two-photon excited fluorescence (TPEF) images, can non-invasively discern distinct, depth resolved metabolic and mitochondrial organization patterns in live 3D engineered epithelial tissues upon the onset of cancerous transformation.

Keywords

biochemistry; biomedical optical imaging; cancer; cellular biophysics; data analysis; fluorescence; image resolution; medical image processing; tissue engineering; two-photon processes; TPEF image; automated image analysis algorithm; depth resolved metabolic organization pattern; depth resolved mitochondrial organization pattern; live 3D epithelial tissue engineering; live 3D squamous epithelia; noninvasive cancerous transformation detection; quantitative optical biomarker; two-photon excited fluorescence image; Biochemistry; Cancer; Fluorescence; Measurement; Optical imaging; Organizations; Three-dimensional displays; biomedical imaging; cancer; engineered epithelial tissues; metabolism; two-photon laser scanning fluorescence microscopy;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioengineering Conference (NEBEC), 2014 40th Annual Northeast

Conference_Location

Boston, MA

Type

conf

DOI

10.1109/NEBEC.2014.6972909

Filename

6972909