A low cost environmental chamber for multiplexed cancer biosensor arrays

A low cost environmental chamber for supporting multiplexed live cell diagnostic and therapeutic testing is presented. The chamber is designed to be compatible with standard twenty-four and ninety-six well plates. In addition, an insert was designed to allow the chamber to interface to a micro-fluidic shear flow sensor. The chamber provides a low cost alternative to microscope enclosure and allows multiplexed optical and electrical impedance measurements to be performed on cells as a continuous function of time. The results of this work demonstrates the potential to reduce the barrier to entry to investigators performing multiplexed optical and impedance measurements. The ability to simultaneously compare optical microscopy images with cellular micro-impedance and other biosensor readings significantly improve the capacity to diagnosis and study new and existing pharmaceutical agents.