Near infrared fluorophore selection for lifetime-based FRET imaging

Author

Sinsuebphon, Nattawut ; Lingling Zhao ; Intes, Xavier ; Bevington, Travis ; Ken, Abe ; Barroso, Margarida

Author_Institution

Dept. of Biomed. Eng., Rensselear Polytech. Inst., Troy, NY, USA

fYear

2014

fDate

25-27 April 2014

Firstpage

1

Lastpage

2

Abstract

Near-infrared fluorophore pairs were comparatively studied to perform quantitative lifetime-based FRET imaging. Comparison in terms of donor lifetime reduction, relative brightness and quantitative estimation of the FRETing donor fraction were carried out using a transferrin based assay applied to breast cancer cells in vitro experiments. The best pair, as defined as the brightest and leading to accurate estimation of FRETing component is identified as the most suitable for in vitro and in vivo pre-clinical application.

Keywords

biomedical optical imaging; cancer; cellular biophysics; fluorescence; infrared imaging; molecular biophysics; proteins; FRETing donor fraction; breast cancer cells; donor lifetime reduction; in vitro preclinical application; in vivo preclinical application; near-infrared fluorophore pairs; near-infrared fluorophore selection; quantitative estimation; quantitative lifetime-based FRET imaging; relative brightness; transferrin based assay; Biomedical imaging; Breast cancer; Brightness; Fluorescence; In vitro; In vivo; FRET; lifetime imaging; time-resolved imaging; transferrin;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioengineering Conference (NEBEC), 2014 40th Annual Northeast

Conference_Location

Boston, MA

Type

conf

DOI

10.1109/NEBEC.2014.6972941

Filename

6972941