Title :
Maximum imaging depth improvement with third-harmonic generation microscopy in turbid tissues
Author :
Yildirim, Muhammed ; Ferhanglu, Onur ; Ben-Yakar, Adela ; Durr, Nicholas
Author_Institution :
Dept. of Mech. Eng., Univ. of Texas at Austin, Austin, TX, USA
Abstract :
Vocal fold scarring is one of the major cause of voice disorders yet there is no reliable treatment. We hypothesized to solve this problem by creating sub-epithelial voids to localize biomaterials to restore the pliability of scarred vocal folds. In order to guide this precise surgery, there is a need for performing deep tissue imaging. Here, we present an ex-vivo study to investigate maximum imaging depth improvement of third-harmonic generation (THG) microscopy in a turbid tissue. We observed 2.5 times improvement with THG microscopy at 1552 nm wavelength compared to two-photon microscopy at 776 nm.
Keywords :
biological tissues; biomedical materials; biomedical optical imaging; medical disorders; optical harmonic generation; optical microscopy; speech; surgery; turbidity; two-photon processes; THG microscopy; biomaterials; deep tissue imaging; maximum imaging depth improvement; scarred vocal fold pliability; sub-epithelial voids; surgery; third-harmonic generation microscopy; turbid tissues; two-photon microscopy; vocal fold scarring; voice disorders; wavelength 1552 nm; wavelength 776 nm; Erbium-doped fiber lasers; Measurement by laser beam; Microscopy; Scattering; Surgery; Temperature measurement; nonlinear microscopy; third-harmonic generation microscopy; turbid tissue; two-photon microscopy; ultrafast lasers; vocal fold scarring;
Conference_Titel :
Bioengineering Conference (NEBEC), 2014 40th Annual Northeast
Conference_Location :
Boston, MA
DOI :
10.1109/NEBEC.2014.6972989
