Title :
MEMS-based sonoporation devices for intratumoral gene therapy
Author :
Siu, T. ; Trivedi, D.M. ; Chiao, M. ; Rohling, R.
Author_Institution :
Dept. of Mech. Eng., British Columbia Univ., Vancouver, BC, Canada
Abstract :
This paper presents a miniature device for intratumoral delivery of oligonucleotides (ASO). A PZT plate (1 cm2 × 0.75mm) is used to transfect cells using cavitation-induced sonoporation. Both human umbilical vein endothelial cells (HUVEC) and human prostate cancer cells (PC3) are investigated in vitro. Preliminary results show that after cavitation-induced sonoporation, the transfection rate for HUVEC increases by 10% compared to controls (p<0.01). For PC3, the transfection rate increases by 7% compared to controls (p<0.01).
Keywords :
biomedical equipment; biomedical ultrasonics; cavitation; cellular biophysics; genetics; micromechanical devices; patient treatment; 0.75 mm; 1 cm; HUVEC; MEMS-based sonoporation devices; PC3; PZT plate; cavitation-induced sonoporation; cell transfection; human prostate cancer cells; human umbilical vein endothelial cells; intratumoral gene therapy; oligonucleotides; Actuators; Biomembranes; Cancer; Cells (biology); DNA; Gene therapy; Humans; In vitro; Medical treatment; Ultrasonic imaging;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems, 2005. Digest of Technical Papers. TRANSDUCERS '05. The 13th International Conference on
Print_ISBN :
0-7803-8994-8
DOI :
10.1109/SENSOR.2005.1496394
